| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Bacillus smithii | AATGTGGCTGGCGTTCCA | ATTCCCAGCACTCCAGCA | 59.88 | 58.50 | 279 | 135 |
100.00%
|
100.00%
|
| Primer 22 | Bacillus smithii | TGCCGATGCTTCGTTTTGC | GCATTCCAGTTGACGGTGC | 60.08 | 59.79 | 250 | 135 |
100.00%
|
100.00%
|
| Primer 23 | Bacillus smithii | AATGTGGCTGGCGTTCCA | TCCCAGCACTCCAGCAGTA | 59.88 | 60.23 | 277 | 133 |
100.00%
|
100.00%
|
| Primer 24 | Bacillus smithii | TCACGCGTTGTCAGCAGT | TGCCGATACCAGCCTTGTC | 59.89 | 59.78 | 171 | 132 |
100.00%
|
100.00%
|
| Primer 25 | Bacillus smithii | TCACGCGTTGTCAGCAGT | TGCCGATACCAGCCTTGT | 59.89 | 58.60 | 171 | 132 |
100.00%
|
100.00%
|
| Primer 26 | Bacillus smithii | TCACGCGTTGTCAGCAGT | CCCGATTGTCCATGCAAGC | 59.89 | 59.57 | 193 | 132 |
100.00%
|
100.00%
|
| Primer 27 | Bacillus smithii | GGTGGCCATTTTGGGTTGG | TCAAACCGATCCCGACAGC | 59.63 | 60.08 | 173 | 132 |
100.00%
|
100.00%
|
| Primer 28 | Bacillus smithii | TGCTGCGGAAATGGCTGA | TCCTTTTCCACGCACGGA | 59.97 | 59.18 | 298 | 132 |
100.00%
|
100.00%
|
| Primer 29 | Bacillus smithii | TGCGCTGCCAATCCATCA | TTCAGGCTGGGGTTCGATG | 60.05 | 59.70 | 277 | 132 |
100.00%
|
100.00%
|
| Primer 30 | Bacillus smithii | TGCGCTGCCAATCCATCA | GGCTGGGGTTCGATGAACT | 60.05 | 59.70 | 273 | 132 |
100.00%
|
100.00%
|